| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Sporting Nebraska FC Sporting Nebr B ECNL 2006 |
03036-0011B7U-0055 | Mark Heath-Preston |
| A2 | Omaha United Soccer Club Omaha United SC 2006B Black |
03133-0011B7U-0009 | Jovan Sudar |
| A3 | Villarreal Nebraska Academy VNA 2006 Elite Boys |
03131-0011B7U-0004 | Jorge Sambataro |
| A4 | Omaha United Soccer Club Omaha United SC 2006B White |
03133-0011B7U-0008 | Randall Esquivel |
| B1 | Interstate Soccer Club ISC 2006B |
04058-0011B7U-0001 | Osbin Rogel |
| B2 | Dreamers FC DFC Aztecs 06 NPL |
03134-0011B7U-1774 | Ricardo Noyola |
| B3 | United Association Football UAF 2006B Blue |
03001-0011B7U-0012 | Steve Monzu |
| B4 | Gretna Elite Academy GEA ECNL B2006 |
02077-0011B7U-0060 | Alex Vazquez |