| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Sporting Nebraska FC Sporting Nebr B ECNL 2007 |
03036-0011B6U-0051 | Karl Ostrand |
| A2 | Sporting Nebraska FC Sporting Nebr B LNK Elite 2007 |
03036-0011B6U-0054 | James Beckmann |
| A3 | Evolution Soccer Club EVO IFA U16 PRIMERO BP |
03099-0011B6U-0154 | Arturo Ochoa |
| A4 | Elkhorn Athletic Association Nebraska Roots 2007B I |
03004-0011B6U-0031 | Marcus Schademann |
| B1 | Evolution Soccer Club EVO IFA U16 PRIMERO RG |
03099-0011B6U-0153 | Jonathan Castro |
| B2 | Interstate Soccer Club ISC 2007B Blue |
04058-0011B6U-0003 | Jenny Amundson |
| B3 | Sporting Nebraska FC Sporting Nebr B NPL 2007 |
03036-0011B6U-0052 | William Taylor |
| B4 | Gretna Elite Academy GEA ECNL B2007 |
02077-0011B6U-0063 | Chris Dunford |