| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Villarreal Nebraska Academy VNA HS Boys 2007 Elite |
03131-0011B7U-0004 | Jorge Sambataro |
| A2 | Iowa - Council Bluffs Soccer Club CBSC Competitive U16B |
0O002-0012B6U-0011 | Jerry Orozco |
| A3 | Dreamers FC DFC Aztecs 08 Blue |
03134-0012B6U-1797 | Jorge Ortega |
| A4 | Elkhorn Athletic Association Nebraska Roots 08/09 Black |
03004-0012B6U-1347 | Brandon Roarty |
| A5 | Elkhorn Athletic Association Nebraska Roots 08/09 Red |
03004-0012B6U-1346 | Brandon Roarty |
| A6 | Omaha United Soccer Club Omaha United SC 2008B White |
03133-0011B6U-0003 | Shad Steffen |
| A7 | Sporting Nebraska FC Sporting Nebr B Gold 2008 |
03036-0011B6U-0049 | Jonathan Iberg |