| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Azzurri Soccer Club Azzurri Italia |
03065-0011B7U-0004 | Martin Alvarez |
| A2 | Iowa - Council Bluffs Soccer Club CBSC Competitive U17B |
0O002-0011B7U-0012 | Adam Sanchez |
| A3 | Fremont Soccer Club FSC United Gold 2007/08 |
04054-0011B7U-0021 | Joel Barcenes |
| A4 | Gretna Elite Academy GEA Gold 07B |
02077-0012B7U-0115 | Callum Goldsmith |
| A5 | Interstate Soccer Club ISC 2007B Orange |
04058-0011B7U-0003 | Sergio Alvarez |
| A6 | Sporting Nebraska FC Sporting Nebr B Gold 2007 |
03036-0011B7U-0053 | Rodrigo Retana-Aguilera |
| A7 | Sporting Nebraska FC Sporting Nebr B LNK Elite 2008 |
03036-0011B6U-0050 | Ben Damewood |
| A8 | Omaha Housing Authority OHA SFA Forest 2007/2008/2009 |
03082-0011B7U-1112 | Osuman Issaka |
| A9 | Dreamers FC DFC Aztecs 07 |
03134-0011B7U-1795 | Arturo Valenzuela |