| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Azzurri Soccer Club Azzurri Elite |
03065-0012B6U-1237 | Andrew Rogers |
| A2 | z-Sparzat Soccer Club Sparzat U-S MEX - B16U |
03081-0012B6U-0047 | Eduardo Galvan |
| A3 | z-Sparzat Soccer Club Sparzat Raptors - B16U |
03081-0011B6U-0049 | Fernando Romero |
| A4 | z-South Sioux City Soccer Club SSC Diablos Black 05 - B16U |
04056-0011B6U-0012 | Andy Reyes |
| A5 | Villarreal Nebraska Academy VNA 2005 Elite Boys |
03131-0011B6U-0014 | Edgar Ramirez |
| A6 | Papillion Soccer Club PSC 2005B Maroon |
02016-0011B6U-0023 | Christopher Sparks |
| A7 | Hawks FC Hawks FC 2005 |
0N135-0011B6U-0006 | Dave Rus |
| A8 | Sporting Nebraska FC SOFC B GOLD 05 |
03036-001OHB6U-2192 | Naser Alsharif |
| A9 | Elkhorn Athletic Association NE Roots 2005B I |
03004-0011B6U-1066 | Chace Hutchison |
| A10 | Gretna Elite Academy GEA NPL 06B |
02077-0011B5U-0039 | Alex Vazquez |