| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lex Soccer Unifut Lex Manchester |
06130-0011B3U-0001 | Artemio Munoz |
| A2 | Dreamers FC DFC MPA NPL 08B |
03134-0011B3U-1766 | Kenan Sahuric |
| A3 | Sporting Nebraska FC SOFC B ELITE 08 |
03036-001EOB3U-2098 | Matthew Swartz |
| A4 | Heartland FC HFC Celtic 2008 |
05085-0011B3U-0007 | Monty RitchieDUP |
| A5 | Gretna Elite Academy GEA NPL 08B |
02077-0011B3U-0029 | Logan Mendez |
| A6 | z-Infinite Sports World ISW Samba |
03124-0011B3U-0001 | Jeff Oksman |
| A7 | Nebraska FC NFC Blue 2008 |
03062-0011B3U-0130 | Jonah Garbin |
| A8 | Elkhorn Athletic Association NE Roots 2008B I |
03004-0011B3U-1060 | Ted Anderson |
| A9 | Sporting Nebraska FC SOFC B ELITE 09 |
03036-001EOB2U-2150 | Mike Dean |