| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Sporting Nebraska FC Sporting Nebr G Academy Celtic 2015 |
03036-0012G9U-2296 | Matt Bragg |
| A2 | Gretna Elite Academy GEA Academy Pre-ECNL 15G |
02077-0012G9U-0089 | Jono Callahan |
| A3 | Gretna Elite Academy - Lincoln GEA Lincoln Gold 15G |
02077-0023G9U-0015 | Annastasia Farlow |
| A4 | Sting Soccer Sting NE 15G Nelson |
03137-0012G9U-0100 | Caitlin Nelson |
| A5 | United Association Football UAF 2015G Blue |
03001-0012G9U-0022 | William Kirby |
| A6 | Blair Soccer Club Blair FC Grizzlies |
04019-0013G9U-1075 | Andrew Anderson |
| A7 | Omaha United Soccer Club Omaha United SC 2015G Black |
03133-0012G9U-0076 | Alissa Kuhl |
| A8 | Bennington Soccer Club BENSC Bolts 9G (S) |
03057-0012G9U-0065 | Darrin Mercer |