| Group | Club/Team Name | Team ID | Head Coach |
| A1 | 402 Development Academy 402 DA 2015 Girls |
03136-0012G9U-0014 | Hunter Isley |
| A2 | Gretna Elite Academy GEA Academy Pre-ECNL 16G |
02077-0012G9U-0123 | Zack Brennan |
| A3 | Sporting Nebraska FC Sporting Nebr G Academy Rangers 2015 |
03036-0012G9U-2371 | Keri Leece |
| A4 | Bennington Soccer Club BENSC Bearcats 9G (S) |
03057-0012G9U-0064 | Andrew Stappert |
| A5 | Sting Soccer Sting NE 15G Nelson White |
03137-0012G9U-0114 | Caitlin Nelson |
| A6 | Sting Soccer Sting NE 15G Sutton |
03137-0012G9U-0121 | Abigail Sutton |
| A7 | Sporting Nebraska FC Sporting Nebr G Pre Acad Ajax 2016 |
03036-0013G8U-2293 | Edward Brown |
| A8 | Omaha United Soccer Club Omaha United SC 2015G White |
03133-0012G9U-0075 | Alissa Kuhl |
| A9 | Iowa - Council Bluffs Soccer Club CBSC U9G Black |
0O002-0013G9U-0026 | Chris Olson |
| A10 | Omaha United Soccer Club Omaha United SC 2016G Black |
03133-0012G8U-0079 | Brianna Agilar |