| Group | Club/Team Name | Team ID | Head Coach |
| A1 | United Association Football UAF 2016B Blue |
03001-0013B9U-0004 | Ian McBride |
| A2 | Sporting Nebraska FC Sporting Nebr B Acad River Plate 2016 |
03036-0012B9U-2410 | Jeremy Forster |
| A3 | Evolution Soccer Club EVO-U9B-Black-BK-2016 |
03099-0012B9U-0023 | William Koeppen |
| A4 | Gretna Elite Academy GEA Academy Pre-ECNL 17B II |
02077-0012B8U-0149 | Armand Garcia |
| A5 | Gretna Elite Academy GEA Academy Gold 16B I |
02077-0012B9U-0129 | Zachary Walsh |
| A6 | Gretna Elite Academy GEA Academy Gold 16B II |
02077-0012B9U-0130 | Zachary Walsh |
| A7 | Omaha United Soccer Club OUSC 2016B WHITE |
03133-0012B9U-0116 | Oscar Castro |
| A8 | 402 Development Academy 402 DA 2016B Gold |
03136-0013B9U-0011 | Jose Hernandez |