| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Elkhorn Athletic Association EAA NE ROOTS 08/09B I |
03004-0011B7U-0013 | Cesar Herrera |
| A2 | United Futbol Academy UFA DSM 2008 B |
0422-01SB08-0001 | Carlos Anaya |
| A3 | Lincoln Surf Lincoln Surf 08B Premier |
03134-0011B7U-1821 | Orlando Quiroz-Espinoza |
| A4 | 402 Development Academy 402 DA 2008B Premier |
03136-0011B7U-0047 | Bradley Wells |
| A5 | 402 Development Academy 402 DA 2009B Premier |
03136-0011B6U-0043 | Kenan Sahuric |
| A6 | Sporting Nebraska FC Sporting Nebr B ECNL 2009 |
03036-0011B6U-2374 | Mike Dean |
| A7 | Sporting Nebraska FC Sporting Nebr B ECNL-RL 2008 |
03036-0011B7U-0047 | Ryan Kruse |
| A8 | Sporting Nebraska FC Sporting Nebr B LNK Elite 2008 |
03036-0011B7U-0050 | Ben Damewood |
| A9 | Omaha Surf Omaha Surf 2008B Black |
02016-0011B7U-0042 | Christopher MacDonald |
| A10 | International Soccer Academy INTER- B17U |
05084-0011B7U-0009 | Jose Marez Ochoa |
| A11 | Impact Football Academy IFA 2008 PRIMERO VAZQUEZ |
03138-0011B7U-0003 | Luis Vazquez |
| A12 | Gretna Elite Academy GEA ECNL-RL 08B |
02077-0011B7U-0065 | Tobias Maertzke |