| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Blair Soccer Club Blair FC Madrid |
04019-0012G2U-1079 | Melanie Wimer |
| A2 | Iowa - Council Bluffs Soccer Club CBSC U12G |
0O002-0012G2U-0021 | Aaron Tatman |
| A3 | Columbus Soccer Club CSC Storm 2012G |
04017-0012G2U-2046 | Jason Beiermann |
| A4 | Fremont Soccer Club Moxie Blue NW Omaha |
04054-0012G2U-0227 | Amy Tilley |
| A5 | Gretna Elite Academy GEA White 12G |
02077-0013G2U-0085 | Jamison Parkhill |
| A6 | Lincoln Spirit Soccer Club LSC Pulse 2012 Red |
03024-0012G2U-3720 | Mike Bohling |
| A7 | Sting Soccer Sting NE 12G Sutton |
03137-0012G2U-0082 | Abigail Sutton |